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Publications Authored by Jean Chang Cells were transfected by calcium phosphate precipitation, as described (6). All transfections included 0.2 μg of p6RlacZ (19) for normalization of transfection efficiency. The radioactive band associated with a polypeptide of Mr 20 kD is labeled p20, whereas that which comigrates with Bax is designated p21 Bax. At 16 h posttransfection, the medium was replaced with phenol red-free DMEM containing 10% charcoal-stripped FBS. Six hours later, the cells were plated onto poly- l-lysine-coated coverslips and incubated overnight in phenol-free DMEM…
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